pH induced secondary structural change of BL.

<p>(A) Far-UV CD spectra of BL at pH 7.4 (-▪-), pH 2.0 (-▴-), pH 0.8 (-▾-) and 6 M GdnHCl (-♦-). (B) The change in mean residual elipticity (MRE) of BL at 218 nm was plotted with respect to pH. (C<b>)</b> Near-UV CD spectrum of BL at pH 7.4 (-▪-), and at pH 2.0 (-▴-). For far-UV CD and Near-UV CD measurements protein concentration was take 0.15 and 1.0 mg ml⁻¹. BL was incubated at different pH overnight prior to measurements.