Size exclusion liquid chromatography (HPSEC) representative chromatograms of EPS isolated from biofilm (A) and from spent medium below the biofilms (B) grown in three media.

<p>SYM (—––); Czapek (– – –), and MSgg (- - - - - -). The molecular weight (M) distributions were graphically split into five size fractions. Their chemical composition and relative abundance of polysaccharides (▪), proteins (▪) and nucleic acids (▪) in these size fractions is graphically represented in A1 and B1. Chemical composition of EPS size fractions was determined from RI (refractive index) chromatograms shown in A and B and from UV 280 and 260 nm chromatograms (not shown).