Budding index of agar cultures of <i>S.cerevisiae</i> on different media with and without oxaloacetate – in control and after induction with 4% CO<sub>2</sub>.
- Ilya V. Volodyaev (407685)
- Elena N. Krasilnikova (407686)
- Ruslan N. Ivanovsky (407687)
Publication date
April 2013
DOIAbstract
<p>Medium content:</p><p>A – Minimal medium with glucose (glucose 0,1%+(NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> 0,1%+KH<sub>2</sub>PO<sub>4</sub> 0,1%+MgSO<sub>4</sub> 0,05%+CaCl<sub>2</sub> 0,01%+NaCl 0,01%, pH5,5),</p><p>B – Minimal medium with acetate, without nitrogen (CH<sub>3</sub>COONa 0,1%+KH<sub>2</sub>PO<sub>4</sub> 0,1%+MgSO<sub>4</sub> 0,05%+CaCl<sub>2</sub> 0,01%+NaCl 0,01%, pH5,5).</p><p>Inductor –4% CO<sub>2</sub>, length of induction –120 min. Recipient – <i>S.cerevisiae</i> culture 15 min after inoculation.</p
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