MCT2 is localized in the arcuate nucleus.

<p>(a) Schematic representation of the hypothalamic area shown in b–d. (b) Rat frontal brain section using anti-vimentin (red) and anti-GLUT1 (blue) antibodies, markers of glial cells. (c) MCT2 localization. (d) Rat frontal brain section using anti-vimentin (red), anti-GLUT1 (blue), and anti-MCT2 (green) antibodies. (e–h) MCT2 was observed in cellular membranes (e) of dorsal peri-ventricular arcuate nucleus areas; this area was negative for vimentin (f, h) and GLUT1 (g, h). GLUT1 and vimentin are co-distributed in the apical region of α-tanycytes (h, triple-arrows). (i–l) In the ventral peri-ventricular area, which contains β1-tanycytes, MCT2 was expressed in cellular membranes; this area was negative for vimentin (j) and GLUT1 (k). GLUT1 and vimentin are co-distributed in apical region of β1-tanycytes (arrows). (m–p) MCT2 was observed in the cellular membranes of β1-tanycyte processes in the ventral lateral area of the arcuate nucleus, which was negative for vimentin (n, p) and GLUT1(o, p). AN: arcuate nucleus, III–V: third ventricle, ME: median eminence. Scale bar: (b and e) 150 µm; (c–d and f–u) 50 µm.