Expression profile of <i>sfrp5</i>.

<p><b>A)</b> Expression level of <i>sfrp5</i> as measured by probeset Dr.21012.1.S1 in GI tissue (dark green squares) and non-GI tissue (light green triangles) from 2 through 6 dpf (for details, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062470#pone.0062470-Stuckenholz1" target="_blank">[39]</a>). <b>B)</b> Expression of <i>sfrp5</i> and β-actin by RT-PCR of total RNA isolated at indicated time points. <b>C–O)</b> Whole-mount <i>in situ</i> hybridization showing <i>sfrp5</i> expression in zebrafish embryos at 3 hpf (<b>C</b>), shield stage (<b>D</b>), 8 hpf (<b>E</b>), bud stage (<b>F</b>), early (<b>G</b>), mid (<b>H</b>), and late somitogenesis (<b>I</b>), 24 hpf (<b>J</b>), 32 hpf (<b>K</b>), 2 dpf (<b>L</b>), 3 dpf (<b>M</b>), 4 dpf (<b>N</b>), and 6 dpf (<b>O</b>). Lateral views with animal pole to the top (<b>C</b>–<b>F</b>) or with anterior to the left (<b>G</b>–<b>I</b>, <b>M</b>–<b>O</b>). Dorsal view with anterior to the left (<b>J</b>–<b>L</b>).