Endodermal defects in embryos with altered levels of Sfrp5.

<p>Embryos were injected with 0.5 pmol morpholino against <i>sfrp5</i> (MO; panels B, G, L), 0.5 pmol of the control morpholino (mmMO; panels A, F, K), 100 pg <i>mCherry</i> mRNA (C, H, M), or 100 pg <i>sfrp5</i> mRNA (D, E, I, J, N, O). <b>A</b>–<b>O</b>) Whole-mount <i>in situ</i> hybridization with a probe against <i>foxa1</i> staining embryos at 25 hpf (A–E), 36 hpf (F–J), and 48 hpf (K–O). Dorsal view, anterior to the left. Arrowheads point to the hepatoblast (F–J). <b>P</b>) Bar chart representing distribution of normal and abnormal embryos processed by <i>in situ</i> hybridization, with representative samples shown in A–O. Below each column, numbers of embryos analyzed.