Effect of hyperoxia on lung architecture, BAL total and differential cell counts, BAL total protein concentrations and IL-6 levels in NB MIF KO and MIF TG mice at PN7.

<p>Representative photomicrographs of lung histology (H&E stain) of NB MIF KO, MIF TG and WT control mice exposed to RA or survived 100% O₂ at PN7 (3A). The figures are illustrative of a minimum of 8 animals in each group. Alveolar size, as measured by chord length and septal thickness, confirmed features noted on lung histology (3B and 3C). Each bar represents the mean ± SEM of a minimum of 5 animals. BAL total and differential cell recovery of NB MIF KO, MIF TG and WT control mice exposed to RA or survived 100% O₂ at PN7 (3D and 3E). Each bar represents the mean ± SEM of a minimum of 12 animals. BAL total protein levels of NB MIF KO, MIF TG and WT control mice exposed to RA or survived 100% O₂ at PN7 (3F). Each bar represents the mean ± SEM of a minimum of 4 animals. BAL IL-6 levels of NB MIF KO, MIF TG and WT control mice exposed to RA or survived 100% O₂ at PN7 (3G). Quantitative measurements of RV hypertrophy (3H).^§Each bar represents the mean ± SEM of a minimum of 3 animals. MIF +/+: wild type; MIF −/−: macrophage migration inhibitory factor knock out; MIF TG +: macrophage migration inhibitory factor (over-expressing) transgenic; BAL: bronchoalveolar lavage; IL-6: interleukin-6; RV: right ventricle; LV: left ventricle; IVS: interventricular septum. ^§Note: MIF KO assessments were done at PN6, due to their increased mortality in hyperoxia at PN7. ##<i>P</i><0.0001, #<i>P</i>≤0.01, *<i>P</i><0.05.