Phenotypes and salt tolerance of the transgenic plants.

<p>Salt stressed 3-weeks-old soil grown plants, irrigated with the indicated NaCl solutions every second day up to 14 days. Phenotypes of plants after 14 d of treatment (<b>A</b>)<b>.</b> Dry weight of whole plants measured after 14 days of salt stress. Values are mean ± SE, n = 10, (*) for <i>P≤0.05</i>, (**) for <i>P≤0.01,</i> (***) for <i>P≤0.001</i> or <i>0.005,</i> significantly different from the control (t-test) (<b>B</b>)<b>.</b> Two-weeks-old seedlings of WT and transgenic lines were used for RNA extraction. To provide salt stress, seedlings were treated with 100 mM NaCl for 24 h before RNA isolation. The transcript level of two stress genes was determined by RT-PCR analyses. The stress genes used for the tests were late embryogenesis abundant proteins <i>LEA4-5</i> and Salt overlay sensitive gene <i>SOS3</i> (AF192886) (<b>C</b>)<b>.</b>