5-FU blocks cell cycle progression in G1 phase.

<p>Cell cycle analysis of control (<b>A</b> and <b>B</b>) and 5-FU treated cells (<b>C-G</b>) was evaluated by flow cytometry based on propidium iodide (PI) intercalation into the cellular chromatin (for details see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063177#s2" target="_blank">Materials and methods</a>). Data are presented as relative fluorescence intensity. (<b>H</b>) Cell sorter analysis revealed that the population of cells in the S and G2 phase was remarkably decreased by 5-FU. Values were represented as the means ± standard deviation.