Virus lung titers in vaccinated mice challenged with influenza viruses from H5, H7, H9, H1 and H3 subtypes.

<p>Mouse groups were immunized with H5b, H7H9b, H5b+H7H9, H5b+H7H9b+H1HA, H5b+NP, H7H9b+NP, H5b+H7H9b+NP, H5b+H7H9b+H1HA+ NP, or ΔE1E3 twice at 4 week intervals. Four weeks post-booster inoculation, the mice (5 animals/group) were challenged with one of the following viruses: Egypt/08, TK/VA, G1/99, pH1N1, or X-31. Three days after challenge, the animals were euthanized, and the lung virus titers were determined as described under Materials and Methods. The data are shown as the mean Log₁₀ TCID₅₀ titers ± SD. The detection limit of the lung viral titer was <0.5 Log₁₀ TCID₅₀/ml (indicated as <0.50).</p>*<p>, <i>P</i>≤0.050 and **, <i>P</i><0.010 compared to ΔE1E3 control. H5b, HAd-1203HA-05HA; H7H9b, HAd-H7HA-H9HA; H5b+H7H9b; HAd-1203HA-05HA+HAd-H7HA-H9HA; H5b+H7H9b+H1HA, HAd-1203HA-05HA+HAd-H7HA-H9HA+HAd-H1HA; H5b+NP, HAd-1203HA-05HA+HAd-NP; H7H9b+NP, HAd-H7HA-H9HA+HAd-NP; H5b+H7H9b+NP; HAd-1203HA-05HA+HAd-H7HA-H9HA+HAd-NP; H5b+H7H9b+H1HA+NP; HAd-1203HA-05HA+HAd-H7HA-H9HA+HAd-H1HA+HAd-NP; ΔE1E3, HAd-ΔE1E3; Egypt/08, A/Egypt/3300-NAMRU3/2008 (H5N1)-PR8-IDCDC-RG13; TK/VA, A/turkey/Virginia/2002 (H7N2)-PR8-IBCDC-5; G1/99, A/Hong Kong/1073/1999 (H9N2); pH1N1, A/California/08/2009 (H1N1); X-31, A/Aichi/2/1968 (H3N2)-PR8.