<i>Sema6B</i>, <i>Sema6C</i>, and <i>Sema6D</i> mRNA expression during postnatal retinal development.

<p><i>In situ</i> hybridization was performed using antisense probes specific for <i>Sema6B</i> (<b>A</b>, <b>G</b>), <i>Sema6C</i> (<b>C</b>, <b>I</b>), or <i>Sema6D</i> (<b>E</b>, <b>K</b>) on retina sections from P7 (<b>A</b>–<b>F</b>) and P14 (<b>G</b>–<b>L</b>) postnatal ages of mice. Adjacent P7 (<b>B</b>, <b>D</b>, <b>F</b>) and P14 (<b>H</b>, <b>J</b>, <b>L</b>) retina sections incubated with sense probes for <i>Sema6B</i> (<b>B</b>, <b>H</b>), <i>Sema6C</i> (<b>D</b>, <b>J</b>), or <i>Sema6D</i> (<b>F</b>, <b>L</b>) were shown as references for background staining. <i>Sema6B</i>, <i>Sema6C</i>, and <i>Sema6D</i> are all expressed at these developmental time points. Strong <i>Sema6B</i> expression is found in the GCL and INL at P7 and P14 (<b>A</b>, <b>G</b>). <i>Sema6C</i> is expressed broadly in the INL and sparsely in the GCL (<b>C</b>, <b>I</b>) at P7 and P14. <i>Sema6D</i> is strongly expressed in specific cell types that reside in the GCL and INL close to the OPL at P7 and P14. <i>Sema6D</i> expression is also observed sparsely in the inner INL (<b>E</b>, <b>K</b>). GCL: ganglion cell layer, IPL: inner plexiform layer, INL: inner nuclear layer, OPL: outer plexiform layer, ONL: outer nuclear layer. Scale bar: 50 µm in <b>L</b> for <b>A</b>–<b>L</b>.