Timecourse of transgene expression by primary human melanocytes following transduction with rAAV6.

<p>(A) Variation with time in the percentage of primary human melanocytes expressing eGFP and (B) the levels of transgene expression in eGFP positive cells as assessed by mean fluorescent intensity (mfi) of positive cells. Cells were infected with 10⁵ vector genomes per cell of rAAV6 and eGFP expression assessed by flow cytometry at days 2, 6, 9 (all four donors), 13, 20, and 28 (two donors). Different symbols represent different donors. Data points represent the average of duplicate repeats. (C) The effect of cell division on the loss of eGFP expression. One rapidly dividing melanoma cell line (SKMel23, represented by the triangle symbol) and 2 slower dividing primary melanocyte cultures (represented by a cross [medium pigmented] or open circle [lightly pigmented]) were infected with 10⁵ vector genomes per cell of rAAV6 and eGFP expression assessed by flow cytometry at either days 2, 9 and 16 (SKMel23) or days 9, 16 and 23 (primary melanocytes). Data points represent the average of triplicate samples.