Loss of <i>Apc</i> inhibits Notch signaling in <i>Apc^Foxj1</i> mouse testis.

<p><b>A</b> Representative immunostaining of Ctnnb1 (green) and NICD (red) in control and <i>Apc^Foxj1</i> testes at 12 months are shown. Arrows in control mouse testis indicate NICD^pos spermatogonia stem cells. Arrows in <b><i>Apc^Foxj1</i></b> mouse testis indicate disappearance of NICD^pos signals in Ctnnb1 accumulating spermatogonia. Scale bar: 30 µm. <b>B</b> Representative western blots analysis of total protein from adult control and <i>Apc^Foxj1</i> testes. β-actin was used as control. <b>C</b> Real-time PCR of Notch-target genes in control and <i>Apc^Foxj1</i> testes at 6 month to 12-month-old. Values are fold induction or inhibition compared to c<i>ontrols</i> (arbitrarily adjusted to 1) (n = 3 for each genotype).