Dynein and kinesin mediate distinct steps in vegetal RNA transport.

<p>(A–D) Fluorescently labeled VLE RNA was microinjected into oocytes expressing (A) kinesin-1 rigor mutant (K1r), (B) p150^Glued CC1 (CC1), (C) both K1r and CC1, or (D) no exogenous protein. Representative oocytes are shown with the vegetal pole towards the bottom. Scale bars, 50 µm. (E) Quantification of in vivo interference results for oocytes expressing no exogenous protein (control, <i>n</i> = 167), kinesin-1 rigor (K1r, <i>n</i> = 159), CC1 domain of p150^Glued (CC1, <i>n</i> = 209), and both K1r and CC1 (<i>n</i> = 208). Black bars indicate normal localization, gray denotes cup accumulation, and white bars indicate accumulation of RNA in the lower vegetal cytoplasm. Error bars indicate standard deviation.