Depletion of Kif3a affects sheet migration.

<p>(<b>A</b>) Kif3a-i cells after 48 h with or without Tet were wounded and migration was observed over time (Video S1). Representative phase contrast images after 4 and 10 h. The Kif3a depleted cells appear denser and smaller. Scale Bars: 100 µm. (<b>B</b>) Quantification of migration speed in the presence or absence of Tet of control cells (Luci-i), 2 different Kif3a-i cell lines and rescue cells (details in text). The area (cm²) filled in during migration was measured semi-automatically (cm²/minute). − and+Tet: controls: 160±22% vs. 134±17%; Kif3a-i1: 155±25% vs. 32±7%; Kif3a-i2: 174±11% vs. 58±6%, Rescue: 200±9% vs. 160±14%, n = 3 each (>5 fields of view per n), * P<0.01, “n.s.” not significant.