Adoptive transfer of WT macrophages in TLR9^−/− mice allow normal TLR response of F4/80⁺CD11b⁺ cells after <i>T.cruzi</i> infection.

<p>Representative flow cytometry plots showing (A) IL-12/IL-23p40⁺MHCII⁺CD11c^high and (B, C) IL-12/IL-23p40⁺F4/80⁺CD11b⁺ cells, stimulated or not with CpG DNA (1 µg/ml), from non-infected or infected C57BL/6 WT, TLR9^−/−, TLR2^−/− and TLR9^−/− or TLR2^−/− mice that received WT macrophages (Rec TLR9^−/− or Rec TLR2^−/− mice). (D) Frequencies of IL-12/IL-23p40⁺F4/80⁺CD11b⁺ cells stimulated with CpG DNA (mean ± SD of four mice) isolated from non-infected or infected C57BL/6 WT, TLR9^−/−, and Rec TLR9^−/− mice. **p<0.01 indicates statistical significance when compared the percentage IL-12/IL-23p40⁺F4/80⁺CD11b⁺ cells after stimulation with CpG DNA in infected C57BL/6 WT or Rec TLR9^−/− mice.