Silencing <i>HMGA1</i> expression halts cell growth and induces dramatic changes in cell morphology and gene expression.

<p><b>A</b>) Lentiviral-mediated delivery of shRNA to <i>HMGA1</i> (denoted shHMGA1) results in a marked decrease in <i>HMGA1</i> mRNA and protein in triple negative breast cancer cell lines (MDA-MB-231, Hs578T). <b>B</b>) Proliferation is disrupted in cancer cell lines following silencing of <i>HMGA1</i>. <b>C</b>) Mesenchymal, fibroblast-like cancer cells undergo dramatic morphologic changes within 4 days after treatment with shHMGA1. Striking changes were observed in MDA-MB-231 (top panels) and Hs578T cells (bottom panels). Bar: 50 µm. <b>D</b>) Alterations in EMT genes with silencing of <i>HMGA1</i>. <b>E</b>) Migration and invasion is decreased with silencing of <i>HMGA1</i>. *P<0.05; **P<0.01.