Inhibition of CCL2-induced mechanical hyperalgesia by ROS scavenging in Wistar rats.

<p>Mechanical nociceptive thresholds were quantified before and after (<b>A</b>) i.pl. injection of 1 µg apocynin with 3 µg CCL2 (filled circles) or 3 µg CCL2 alone and (<b>B</b>) i.pl. injection of 1 µg apocynin (filled circles) or its solvent 0.9 % NaCl (open circles) in rats with 4 d CFA inflammation. (<b>C–E</b>) Mechanical nociceptive thresholds were quantified before and after i.pl. injection of 3 µg CCL2 alone with the respective solvent (filled circle) or together with (<b>C</b>) SOD (3 U open circle, 30 U filled triangle, 300 U open triangle), (<b>D</b>) catalase (2–5 U – open circle, 20–50 U – filled triangle, 200–500 U – open triangle), or (<b>E</b>) tempol i.p. (1.5 mg – open circle, 7.5 mg – filled triangle, 15 mg – open triangle) (*p<0.05 versus time point 0 h, Two Way RM ANOVA, Student-Newman-Keuls, n = 6). Data are presented as means ± SEM.