Summary table of qPCR, ddPCR and cdPCR performance for MON810 detection and quantification.

<p>qPCR: data produced in this study, or obtained from the literature, when indicated <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062583#pone.0062583-Burns1" target="_blank">[18]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062583#pone.0062583-EuropeanUnionReferenceLaboratoryforGM1" target="_blank">[22]</a></p><p>ddPCR: data produced in this study.</p><p>cdPCR: data produced on a BioMark System (Fluidigm, South San Francisco) using the 12.765 digital arrays (Fluidigm) and obtained from the literature <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062583#pone.0062583-Corbisier1" target="_blank">[11]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062583#pone.0062583-Burns1" target="_blank">[18]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062583#pone.0062583-Bhat1" target="_blank">[19]</a>.</p><p>Repeatability through the dynamic range: assessed through the coefficient of variation (Cv) of the target copy numbers or the MON810 content between repeats.</p><p>Trueness: assessed through the calculation of the bias between the MON810 content measured and the target MON810 content. * For our study, trueness is indicated only when qPCR and ddPCR results could be compared to a third, independent value (obtained from the CRM provider or proficiency test organizer).</p><p>Time for results/96 well plate: Total time needed from DNA pipetting to the analysis of the results; reaction mixes are already prepared.</p><p>Price/sample if 96-well plate: Price based on material and reagent costs available at NIB, including labor cost.</p><p>N.A.: not evaluated.