Gel filtration chromatography of purified PACT-WT and PACT mutant proteins with siRNA.

<p>Gel filtration chromatography patterns of non-labeled siLuc-36 (300 nM) with PACT-WT (A), PACT- dsRBDmt1 (B), PACT-dsRBDmt2 (C), and PACT-ΔdsRBD3 (D). Lower panels showed the results of Western blot (WB) by anti-myc antibody for detecting PACT proteins in the elution fractions. Histograms below WBs showed the quantified signal densities of PACT proteins detected by WBs. Arrowheads indicate the positions of molecular weight size markers.