Characterization of human BMMSCs.

<p>(A–C) Cells cultured in osteoblast-inductive medium for three weeks, visualized with ALP (A), alizarin red (B) and von Kossa (C) staining to show human BMMSCs that differentiated into osteogenic cells. Scale bar, 100 μm; (D) Chondrogenic differentiation in chondrocyte-inductive medium after three weeks, with the differentiated cells examined via alcian blue Staining. Scale bar, 50 μm; (E, F) Adipogenic differentiation of human BMMSCs induced by adipocyte-inductive medium. One week after induction, intracellular fat droplets were detected via phase-contrast light microscopy (E) and Oil Red O staining (F). Scale bar, 10 μm.