Induced expression of FLP-18 in <i>acr-2(gf)</i> correlates with the onset of convulsions, and high levels of FLP-18 or FLP-1 suppress convulsions.

<p>(A) Quantification of the number of cell bodies in the ventral cord that showed <i>Pflp-18::flp-18::SL2::gfp</i> expression in larval and adult stages. Each dot indicates quantification from one animal. Means are indicated by lines. Error bars indicate SEM. Two independent lines <i>juEx4062</i> and <i>juEx4073</i> were tested. Result from <i>juEx4073</i> is shown. (B) Convulsion of <i>acr-2(gf)</i> was suppressed by expression of <i>Pflp-18::flp-18::SL2::gfp</i> or pan-neuronal expression of <i>flp-1</i>. The suppression by <i>flp-18</i> overexpression was blocked by loss of both <i>npr-1</i> and <i>npr-5</i>, or <i>npr-4</i> and <i>npr-5</i>. The same set of <i>npr</i> mutations did not affect the suppression effect of <i>flp-1</i> overexpression. Mean convulsion frequencies are shown. Error bars indicate SEM. Statistics: ***: <i>p</i><0.001, *: <i>p</i><0.05 by ANOVA and Dunnett's post-hoc test. <i>(+/+)</i> indicates strains with no mutations in any of the neuropeptide receptor genes.