NPR-1, NPR-4, and NPR-5 act together to mediate the effects of neuropeptides on convulsions.

<p>(A–B). Convulsion frequencies of <i>acr-2(gf)</i> combined with loss of function mutations in <i>npr-1(ok1447)</i>, <i>npr-4(tm1782)</i>, <i>npr-5(ok1583)</i> (A) and with <i>flp-1(yn4)</i> (B). (C) Convulsion frequency of animals with cell type-specific expression of <i>npr-1</i> and <i>npr-5</i>. <i>npr-5</i> expression in the muscle rescued the increased convulsion frequency of <i>npr-5(lf); npr-1(lf) acr-2(gf)</i> triple mutant; two independent lines were tested. <i>npr-1</i> expression in GABAergic motor neurons did not significantly rescue the increased convulsion frequency; three lines were tested. All strains contain <i>acr-2(gf)</i>. Mean convulsion frequencies are shown. Error bars indicate SEM. Statistics: ***: <i>p</i><0.001, *: <i>p</i><0.05 by ANOVA and Bonferroni post hoc test. <i>(+/+)</i> indicates strain with no mutations in any of the neuropeptide receptor genes.