The effects of PTE on ROS generation, the GSH level and the GSH/GSSG ratio in lung adenocarcinoma cells treated for 24 h.

<p>(A) To determine the ROS concentration, the cells were loaded with DCFH-DA, and the fluorescence intensity was measured using a microplate fluorescence reader. The fluorescence intensity in the control group was defined as 100%. (B) The intracellular GSH content was measured using a GSH kit. The differences in the GSH level between groups were expressed as a percentage of the control. (C) The GSH and GSSG levels were evaluated by comparison with the standards and were normalized to the protein content. The results are expressed as the GSH/GSSG ratio. The results are expressed as the mean ± SEM, n = 6. **P<0.01 compared with the control group, ^##P<0.01 compared with the PTE 1.5 µM group, ^$$P<0.01 compared with the PTE 3 µM group. PTE, pterostilbene; ROS, reactive oxygen species; GSH, glutathione; GSSG, glutathione disulfide.