Berberine inhibits IFN-γ and TNF-α-induced increases of MLC phosphorylation and MLCK protein expression.

<p>Caco-2 monolayers were treated as described in Fig. 1A. A. Berberine significantly suppressed the increase of phosphorylated MLC expression induced by IFN-γ and TNF-α. *<i>p</i><0.05, compared with control, #<i>p</i><0.05, compared with IFN-γ/TNF-α. Data are representative of five similar experiments. B. Berberine abolished the IFN-γ and TNF-α-caused up-regulation of MLCK protein expression. *<i>p</i><0.05, compared with control, #<i>p</i><0.05, compared with IFN-γ/TNF-α. Data are representative of five similar experiments.