Time course of activation of pro-enzymes to the mature and active form of the wild-type and the mutants of Erv-C.

<p>Aliquots of purified pro-enzymes (10–20 µg) were treated for activation for 0 to 50 minutes to convert into their respective mature forms and the percentage of residual enzyme activities were determined with respect to the maximum activity using an azocasein assay, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062619#s2" target="_blank">Materials and methods</a>.