Knockdown of Miz1 inhibits cell proliferation and tumorigenesis in PZp53^MED1 medulloblastoma cells.

<p>(A) and (B) The rate of cell proliferation was measured in stable scrambled control (Scr) and Miz1 knockdown (#3 and #5) PZp53^MED1 cells using WST-1 (A) and ³H-thymidine incorporation assays (B). Data shown in the graphs represent the mean ± SEM (n = 3; **: <i>p</i><0.001, compared to the Scr sample, t test). (C) and (D) The levels of mRNA expression for Gli1 (C) and Ptch1 (D) were measured using qRT-PCR in control and Miz1 knockdown PZp53^MED1 cells. Data shown in the graphs represent the mean ± SEM (n = 4 for Gli1 and n = 3 for Ptch1; *: <i>p</i><0.05; and **: <i>p</i><0.001, compared to the Scr sample, t test). (E) SCID mice were inoculated subcutaneously with stable control and Miz1 knockdown PZp53^MED1 cells. Image shows the tumors dissected from mice at the end of the experiment. ND represents non-detectable tumor growth around the injection sites. (F) Quantitative representation of tumor weights measured. Data in the graph represent the mean ± SEM (n = 5 for each group; *: <i>p</i><0.05, compared to the Scr sample, t test).