Serum deprivation induced cell death when Bcl-2 function was suppressed.

<p>A: Flow cytometry analysis of SH-SY5Y control cells transfected with negative control, Bcl-2 RNAi #6 and Bcl-2 RNAi #7. The cells were either growing in normal medium or subjected to 12 hrs starvation. B: Quantitative analysis of cell death by flow cytometry Annexin V/PI staining. Cells were either maintained in normal medium or subjected to 12 hrs of starvation prior to cell death analysis. Statistical analysis was either carried with Student t-test or carried out with ANOVA followed by Dunnett t-test (##p<0.01, ###p<0.001, Bcl-2 RNAi #6 + starvation, or #7 + starvation group vs NC+starvation group). C: Nutrient deprivation induced activation of caspase-3 (p19 and p17) and PARP cleavage in SH-SY5Y cells when Bcl-2 was knocked down. The results are representative of three experiments. D: Flow cytometry analysis of SH-SY5Y.control cells treated with DMSO, HA 14-1 and ABT-737. E: Quantitative analysis of cell death by flow cytometry Annexin V/PI staining in SH-SY5Y cells treated with DMSO, HA 14-1 and ABT-737. Cells were either maintained in normal medium or subjected to 12 hrs of starvation prior to cell death analysis. Statistical analysis was either carried out with Student t-test or carried out with ANOVA followed by Dunnett t-test (###p<0.001, HA 14-1 + starvation, or ABT-737 + starvation group vs DMSO + starvation group). For B and E, data represent mean ± SD for combined data from three independent experiments.