Chromosomal map and PCR analysis of the Uridine Monophosphate (UMP) biosynthetic gene cluster in <i>C.bescii</i> DSM 6725 and the spontaneous deletion in <i>pyrFA</i> (JWCB005) locus.

<p>(A) A diagram of the <i>pyr</i> operon region with the 878 bp deletion in the <i>pyrFA</i> ORFs. The line below the diagram indicates the length of the deletion. Bent arrows depict primers used for verification of the structure of the chromosome in the JWCB005 (Δ<i>pyrFA</i>) strain. <i>pyrF</i> and <i>pyrE</i> loci indicated as black color filled arrow and black dashed filled arrow, respectively. (B) Gel depicting PCR products of the <i>pyrFA</i> region in wild type (3.44 kb) compared to the Δ<i>pyrFA</i> (2.52 kb) strain amplified by primers (JH020 and FJ298). (C) Gel depicting the 2.66 kb PCR products of <i>pyrE</i> region in wild type and the Δ<i>pyrFA</i> strain by primers (DC326 and DC331). M: 1 kb DNA ladder (NEB).