L-canavanine aversion is reduced when bitter-sensitive taste neurons express a RNAi construct against Gαo47A or a dominant negative form of Gαo47A.

<p>Two-choice feeding test experiments showing preference index (PI) for the blue solution of different genotypes. Control (white bars) and 30 mM L-canavanine (black bars) indicate that no drug or 30 mM L-canavanine was added to the blue solution, respectively. A complete preference or aversion is indicated by a PI value of 1 or 0, respectively. The down regulation of Gαo47A by RNA interference (Gr66a-Gal4/+;UAS-RNAiGo/+) and the inhibition of Gαo47A by using a dominant negative construct (Gr66a-Gal4/UAS-Go^GDP) reduced the aversion to L-canavanine compared to controls (wild-type, Gr66a-Gal4/+, UAS-RNAiGo/+ and UAS-Go^GDP/+). Note that all genotypes did not show any defect for sugar detection. Error bars indicate SEM. Asterisks indicate significant differences by Unpaired Student's <i>t</i> test (** p<0.01, *** p<0.001).