Etodolac inhibits lung metastasis in a murine syngeneic breast cancer model.

<p><b><b><i>Panel A</i></b><b>:</b></b> Primary tumor volumes were measured at the indicated time point. Feeding with 500 ppm etodolac-containing food started 7 days before tumor cell injection (10⁵ cells) into the right inguinal mammary fat pad of 7-week-old female BALB/c mice and continued until sacrifice. n = 6. <b><i>Panel B</i></b><b>:</b> Metastatic lung nodules on lung surfaces were manually counted and measured in size after resecting lungs from sacrificed mice. Means of a total of three separate counts. <i>p</i><0.05, Mann-whitney U test, n = 6. <b><i>Panel C</i></b><b>:</b> Lung metastasis images obtained by fluorescence microscopy after 4T1_GFP cell implantation (Left panel). Right graph represents quantitatively analyzed fluorescence density measured using Image J software (<i>p</i> = 0.0377, Student's unpaired <i>t</i>-test, <i>n</i> = 3). <b><i>Panel D</i></b><b>:</b> Lung histology by HE staining. Scale bars, left bottom.