Fluvastatin does not affect TNFα release in NK cells.

<p>(A). TNFα, measured by ELISA, present in supernatant of NK cells cultured for 6d+IL2 in medium alone (medium) or solvent of fluvastatin (solvent) or with fluvastatin (10-1.0-0.1 µM) and incubated with K562 HLAI⁻ target cells, at the NK:target cell ratio of 2∶1. (B). TNFα in SN of NK cells, cultured in A, incubated with HLAI+ Jurkat target cells (2∶1 ratio). (C). TNFα in SN of NK cells, cultured as in A, incubated with P815 target cells in the presence of anti-NKG2D mAb to evaluate receptor-mediated TNFα release. In the different panels: K562 (A), Jurkat (B), P815 (C): basal level of cytokine production of tumor target cells. Left column in panel C indicate the basal level of cytokine production of NK cells. Results are expressed as pg/ml and are representative of three independent experiments. In some experiments, the effect of chelation of intracellular calcium with BAPTA-AM on TNFα release was analyzed (B).