Confirmation of BSP knockdown detected by western blot (A) and qRT-PCR (B).

<p>The protein and mRNA levels of BSP are reduced significantly in both 231BO-BSP27 and 231BO-BSP81 cells. 231BO-BSP100 cells show a slight decrease, while 231BO-Scrambled cells show no significant difference from the parental 231BO cells. β-actin is used for control of loading. **p<0.001, significantly different from the 231BO.