Interactions of ClpB with aggregated substrates.

<p>EcClpB (A) or EhClpB (B, C) was incubated with the native (N) or aggregated G6PDH (A, B) or luciferase, Luc (C) in the presence of the indicated nucleotides. The solutions were passed through a 0.1- µm filter and the fractions retained on the filter were analyzed by SDS-PAGE with Coomassie stain. The first lane in (A) shows EcClpB incubated with the native G6PDH and ATPγS, in (B, C) it shows EhClpB incubated with the native G6PDH or luciferase and ATP.