Itu induced cell death in p53-dependent and independent manners.

<p>A. HCT116 cells (p53+/+ and p53−/−) were treated with various concentrations of Itu for 48 hours and the number of cells were measured by Wst-1 assay. *p<0.05 when p53−/− cells were compared to p53+/+ cells. B. Atm+/+ and Atm−/− MEFs were treated with various concentrations of Itu for 48 hours and the number of cells were measured by Wst-1 assay. *p<0.05 when Atm−/− cells were compared to Atm+/+ cells. C. Wild type and p53−/− HCT116 cells were treated with Itu for 24 hours and the number of cells was measured by Wst-1 assay. Itu did not induce cell death when cells were cultured in 0.1% serum or confluent in wild type HCT116 cells. *p<0.05 when compared to untreated cells.