Amyloid-like soluble oligomers or fibrils of elevated human α-synuclein were <i>not</i> detected in ASOTg brain mitochondria and synaptoneurosome fractions.

<p>A. On dots blots, the α-synuclein antibody detected recombinant human α-synuclein in increasing amounts (2.5–100 ng), serving as a monomer positive control (top panel). Aβ₄₂ soluble oligomers and Aβ₄₂ fibrils (200 ng each) served as positive controls for the A11 antibody (middle panel) and the OC antibody (bottom panel) respectively. B. The α-synuclein antibody detected elevated human α-synuclein in ASOTg mitochondrial (mito) and synaptoneurosome (SN) fractions (1 µg) relative to WT fractions (top panel). An SN fraction from <i>Snca^−/−</i>mice (KO) served as a negative control. The A11 and OC antibodies failed to detect endogenous mouse (WT) or elevated human α-synuclein (ASOTg) in all fractions when compared to KO negative control (middle/bottom panels).