The inhibitory effects of inhibitors targeted MAPK/ERK pathway can down-regulate the SLP-2 expression.

<p><b>Aa, Da:</b> Both KYSE510 cells (A) and EC9706 (D) were pretreated with two different ERK1/2 inhibitors (PD98059, 1 µM and U0126, 1 µM) for 3 hours, and then treated with PMA (100 nM) for 15 minutes to analyze the level of phosphorylated ERK1/2 and total ERK1/2. And then around 24 hours to detect SLP-2 expression. <b>Ab, Db:</b> The graph represents densitometry of the results of 3 independent experiments (mean±SD). <b>Ba, Ea:</b> Both KYSE510 cells (B) and EC9706 cells (E) were pretreated with two different AKT inhibitors (LY294002, 1 µM and MK-2206, 1 µM) for 3 hours, and then treated with PMA (100 nM) for 15 minutes to analyze the level of phosphorylated AKT and total AKT. Then, the rest of procedure of detection is similar. <b>Bb, Eb:</b> The graph represents densitometry of the results of 3 independent experiments (mean±SD). <b>Ca, Fa, Ga:</b> Both KYSE510 cells (C) and EC9706 cells (F, G) were pretreated with two different ERK1/2 inhibitors and two AKT inhibitors for 3 hours, and then treated with EGF (10 nM) for 15 minutes to analyze the level of phosphorylated ERK1/2, AKT and total ERK1/2, AKT. And then around 24 hours to detect SLP-2 expression. <b>Cb, Fb, Gb:</b> The graph represents densitometry of the results of 3 independent experiments (mean±SD).