<i>Ex vivo</i> analysis shows the infiltration of CD8+ T cells to brain and glioma.

<p>Panel <b>A</b> shows IHC of fluorescently labeled Pmel-1 CD8+ T cells. Animals were infused with T cells (2×10⁷) and sacrificed for IHC staining two days after cell infusion. Sparse T cells are shown at the arrows. Panels <b>B–C</b> show quantitative flow cytometry of Pmel-1, hgp100-specific T cells in brain cell suspension with CD8+ (<b>B</b>) and CD3+ (<b>C</b>) gating. Panel <b>D</b> shows quantitative flow cytometry of wild-type T cells. Here, PE = Phycoerythrin, FITC = Fluorescein isothiocyanate, and TIL = tumor infiltrating lymphocytes.