DEX pre-treatment and NDV infection cooperatively regulated mRNA expression of the four genes in DCs.

<p>Bone marrow-derived DCs were pre-treated with DEX (10⁻⁶M) for 30 min, and were infected with NDV (MOI = 10) for 6 hours. mRNA levels of top four genes in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063587#pone.0063587.s003" target="_blank">Table S2</a> were measured in the real-time qPCR by using newly designed primers (panel <b>A</b>: <i>IL-10</i> and <i>CLEC4E</i>: genes whose mRNA expression was cooperatively enhanced by DEX pre-treatment and NDV infection, panel <b>B</b>: <i>PTGS2</i> and <i>IFNγ</i> : genes whose mRNA expression was stimulated by NDV infection but repressed by DEX pre-treatment). Their C_t values were normalized with those of <i>GAPDH</i>, and relative mRNA expression (fold changes) was calculated by comparing to the control obtained in the absence of DEX pre-treatment and NDV infection. Bars represent means and standard errors of fold changes of their mRNA expression obtained in three independent experiments. *: p<0.05, **: p<0.01, compared the 2 conditions indicated.