WFA's cell cycle effects are regulated by the skp2-p27^kip1 pathway in RbTCF cells.

<p>(A) Representative images of RbTCFs stimulated to enter the cell cycle after 48 h serum starvation. Cells were stained with vimentin (<i>green</i>) and phalloidin (<i>red</i>) at 24 h post-WFA treatment. WFA's low concentration cell cycle activity does not affect the vimentin-IF network (<i>green</i>). Vimentin-IFs become affected only at higher concentrations (1 µM, <i>arrow heads</i>) and leads to dismantling of the actin fibers (<i>red</i>) that are observed at the periphery of cytoplasm (<i>arrows</i>). (B) Western blot analysis of skp2 and p27^kip1 expression in RbTCF cells at 24 h post-WFA treatment. C) Densitometric quantification of skp2 (<i>black</i>) and p27^kip1 (<i>grey</i>) normalized to β-actin, using ImageJ software. Scale bars: 30 µm at 30× magnification. * P<0.05 WFA vs control.