URI binds PDRG1 through the prefoldin-like domain.

<p>a) Schematic of the seven URI constructs used to map the domains of interaction between URI and PDRG1. The known URI domains are reported and the numbers on top indicate the corresponding amino-acid number. b) An empty vector (vect.), PDRG1 (gray box; 5 µg) and the different URI constructs were transfected into HEK293 cells (10 µg total DNA transfected). URI deleted of the prefoldin domain is labeled PFD. 48 hrs after transfection cells were lysed. Part of the lysate was used as INPUT (left panel) and another part (≈1 mg of proteins) was used to immunoprecipitate URI using FLAG antibodies. The immunocomplexes were analyzed by Western blot using the indicated antibodies. Arrowheads indicate the URI deletions/truncations.