Transport studies with sulfated steroid hormones.

<p>(<b>A</b>) SOAT-HEK293 were incubated with 10 µM non-radiolabeled estrone-3-sulfate, DHEAS, β-estradiol-3-sulfate, and androstenediol-3-sulfate in the presence (black bars) or absence (open bars) of sodium over 10 min at 37°C. Cells lysates were analyzed by LC-MS-MS in order to determine the absolute cell-associated amount of the steroid sulfate molecules in their intact forms. (<b>B</b>) Transport studies with 10 µM [³H]estrone-3-sulfate on stably transfected SOAT-, OATP6A1-, OATP1C1-, and OSCP1-HEK293 cells in the presence and absence of sodium. Non-transfected HEK293 cells served as a control. In contrast to SOAT, OATP6A1, OATP1C1, and OSCP1 showed no significant transport function for E1S.