Sequence and structure of the synthetic IN genes.

<p>Amino acid sequences of the consensus HIV-1 clade A integrase (IN_a), its inactive variant containing mutation in the active site D64V (IN_in), and inactive variant with mutations conferring resistance to elvitegravir H51Y, E92Q, S147G, E157Q, K160Q (IN_in_e3), all with Met-Gly dipeptide on the N-terminus (<b>A</b>); Schematic representation of the structure of the synthetic genes. The following regions are indicated: IN ORF including the Met-Gly dipeptide, 5′- and 3′-end nucleotide flanks with <i>BamHI</i> and <i>EcoRI</i> restriction sites (<b>B</b>).