IFN-γ/IL-2 Fluorospot assay of the splenocytes of mice immunized with IN gene variants.

<p>The results of IFN-γ/IL-2 Fluorospot performed on splenocytes of mice immunized with plasmids encoding consensus IN (IN_a), inactivated consensus IN (IN_in), inactivated consensus IN with mutations conferring resistance to elvitegravir (IN_in_e3), or empty vector. Splenocytes were stimulated <i>in vitro</i> with a Luc-derived peptide (LUC), and individual or pooled IN-derived peptides (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062720#pone-0062720-t003" target="_blank">Table 3</a>) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062720#s4" target="_blank">Methods</a>. IN-specific <i>in vitro</i> secretion of IFN-γ (<b>A</b>), IL-2 (<b>B</b>), and dual secretion of IFN-γ/IL-2 (<b>C</b>). Responses represent the average number of signal-forming units (sfu) per mln cells in two independent experiment runs, each done in duplicate,+SD.