<p>(A) Intracellular level of cAMP. Cells (wild-type) were inoculated in SC-glucose medium and grown to mid-log phase, drug was added, and the samples were incubated for the indicated times. cAMP was extracted from cells and quantified using an immunoassay (Cell Signaling). Values are the mean ± SD of the four independent experiments. (B) Doubling time. Cells (wild-type) were inoculated into SC-glucose containing triclabendazole (5 μM) or DMSO with added nucleotide (cAMP, AMP or ATP) and incubated with shaking at 30°C. Doubling times are the mean ± SD of the three independent experiments. *, <i>p</i><0.005. (C) Msn2-GFP localization. Cells expressing Msn2-GFP were grown to mid-log phase, and then triclabendazole (5 μM) or triclabendazole (5...
<p><b>A)</b> HEK293 cells were co-transfected with GPR3-HA and pcDNA or ...
<p>T84 cells were incubated for an hour with DMSO (vehicle), diclofenac (200 µM), forskolin (20 µM) ...
<p>A: Epac1-camps-expressing cells were stimulated by 25 mM glucose and changes in [cAMP]<sub>c</sub...
<p>(A) Triclabendazole activates nuclear localization of the Msn2. Msn2-GFP were pre-grown to A<sub>...
<p>(A) Doubling time. Cells (wild-type, WT, or <i>rim15</i>Δ) were inoculated in SC-glucose medium w...
<p>Triclabendazole (5 μM) or vehicle was added to diluted cells in SC-glucose medium, and then cells...
Investigations are reported pertinent to the physiologic relevance of the antagonism between cAMP an...
<p><b>A-B).</b> HEK293 cells were transfected with GPR3-HA WT and 4 hr later treated with 5 µM Bis I...
<p>(A) Triclabendazole, nocodazole and benomyl. (B) Spindle formation. Cells expressing Tub1-GFP wer...
<p>(A) Expression of Mitf in B16-F0 cells after 6 h of treatment with α-MSH (0.1 µM) in presence or ...
Cyclic AMP concentrations have been measured in a number of different cell types under a variety of ...
The Ras-adenylyl cyclase-protein kinase A nutrient-sensing pathway controls metabolism, proliferatio...
The Ras-adenylyl cyclase-protein kinase A nutrient-sensing pathway controls metabolism, proliferatio...
<p>A) Effect of db-cAMP treatment on intracellular cAMP levels in Mtb. <i>In vitro</i> grown culture...
<p>(A) Carbon source utilization. Cells were inoculated in SC-glucose, -galactose, or -glycerol medi...
<p><b>A)</b> HEK293 cells were co-transfected with GPR3-HA and pcDNA or ...
<p>T84 cells were incubated for an hour with DMSO (vehicle), diclofenac (200 µM), forskolin (20 µM) ...
<p>A: Epac1-camps-expressing cells were stimulated by 25 mM glucose and changes in [cAMP]<sub>c</sub...
<p>(A) Triclabendazole activates nuclear localization of the Msn2. Msn2-GFP were pre-grown to A<sub>...
<p>(A) Doubling time. Cells (wild-type, WT, or <i>rim15</i>Δ) were inoculated in SC-glucose medium w...
<p>Triclabendazole (5 μM) or vehicle was added to diluted cells in SC-glucose medium, and then cells...
Investigations are reported pertinent to the physiologic relevance of the antagonism between cAMP an...
<p><b>A-B).</b> HEK293 cells were transfected with GPR3-HA WT and 4 hr later treated with 5 µM Bis I...
<p>(A) Triclabendazole, nocodazole and benomyl. (B) Spindle formation. Cells expressing Tub1-GFP wer...
<p>(A) Expression of Mitf in B16-F0 cells after 6 h of treatment with α-MSH (0.1 µM) in presence or ...
Cyclic AMP concentrations have been measured in a number of different cell types under a variety of ...
The Ras-adenylyl cyclase-protein kinase A nutrient-sensing pathway controls metabolism, proliferatio...
The Ras-adenylyl cyclase-protein kinase A nutrient-sensing pathway controls metabolism, proliferatio...
<p>A) Effect of db-cAMP treatment on intracellular cAMP levels in Mtb. <i>In vitro</i> grown culture...
<p>(A) Carbon source utilization. Cells were inoculated in SC-glucose, -galactose, or -glycerol medi...
<p><b>A)</b> HEK293 cells were co-transfected with GPR3-HA and pcDNA or ...
<p>T84 cells were incubated for an hour with DMSO (vehicle), diclofenac (200 µM), forskolin (20 µM) ...
<p>A: Epac1-camps-expressing cells were stimulated by 25 mM glucose and changes in [cAMP]<sub>c</sub...