FMPs express MyoD in developing limbs.

<p>(<b>A–F</b>) Immunohistochemistry of FMP cells on longitudinal sections of limbs at E16.5 for GFP (A,D), MyoD (B,E) and merged with DAPI (C,F). MyoD-positive (A–C) and -negative (D–F) FMPs are shown. Scale bar = 10 µm. (<b>G</b>) Percentage of MyoD-positive and MyoD-negative cells in (Pax3)GFP+ cells in limbs at E16.5 with data reported as the mean and s.d. of three fetuses. (<b>H–M</b>) Immunocytochemistry of freshly isolated FMPs (H–J) and SCs (K–M) for GFP (H,K), MyoD (I,L), and merged with DAPI (J,M). Arrows in H–J indicate MyoD-positive FMPs. Scale bar = 20 µm. (<b>N</b>) Percentage of MyoD-positive and MyoD-negative cells of freshly isolated FMPs and SCs (<i>n = </i>100 cells par condition). FMPs, fetal skeletal muscle progenitors; SCs, satellite cells.