Bck2 localizes to the promoters of M/G₁, G₁/S and G₂/M genes.

<p>(A) Bck2 localization to M/G₁ genes depends on ECBs. WT strain (BY2125; W303) or a strain containing mutated ECB elements in the <i>CLN3</i> and <i>SWI4</i> promoters (BY2680; <i>cln3</i>(ecb)<i>swi4</i>(ecb)) was transformed with a pGAL-<i>BCK2</i>-FLAG plasmid and grown separately in raffinose- (non-inducing conditions) or galactose-containing medium (inducing conditions) to mid-log phase. Cultures were harvested and anti-FLAG ChIPs were analyzed for <i>CLN2</i>, <i>CLN3</i> and <i>SWI4</i> promoter DNA by Q-PCR. The Y-axis measures enrichment of promoter DNA for the target gene indicated relative to enrichment of non-promoter DNA from an untranscribed region of chromosome II. (B) Bck2 localization to the <i>CLN2</i> promoter is reduced when SCBs or Mcm1-binding sites are mutated. Vector and pGAL-BCK2-FLAG were transformed into WT (GC46), and strains with 3 SCBs mutated (yLB76-scb*) and 2 Mcm1-binding sites mutated (yLB76-mcm1*), cells were grown in inducing conditions, and Bck2-Flag localization to the <i>CLN2</i> promoter was analyzed by Q-PCR. (C) Bck2 localizes to the <i>CLB2</i> promoter. WT cells containing vector or pGAL-BCK2-FLAG were grown in inducing conditions and Bck2-Flag localization to the <i>CLN3</i> and <i>CLB2</i> promoter was analyzed by ChIP.