<i>B.</i> pseudomallei rapidly induces COX-2 and PGE₂ production by macrophages.

<p><b>A</b>) Bone-marrow derived macrophages (BMDM) were treated with viable <i>B. thailandensis</i> (Bt), <i>B. pseudomallei</i> (Bp) or heat-inactivated Bp (iBp) at MOI 1 and COX-2 mRNA expression was measured by RT-PCR. <b>B</b>) COX-2 enzyme was detected by Western blot in BMDM infected with Bp at MOI 1. <b>C</b>) BMDM were treated with Bp at MOI 0.1 or 1 and iBp at MOI 1 and PGE₂ was measured in culture supernatants by ELISA. The data represent biological triplicates per time point. Error bars represent the standard deviation (SD). Statistical significance was determined using a two way ANOVA with Bonferroni post-test. *p<0.05, *** p<0.001. Data is representative of two independent experiments.