Glycoprotein detection by Periodic Acid Schiff’s (PAS) staining.

<p>Lane 1: kidney bean extract, lane 2: purified 31 kDa protein (A). Immunoblot of purified protein after periodate treatment. The 31 kDa protein was electro-transfered onto nitrocellulose membrane and then periodate oxidation was done. The strip was washed, blocked with 3% defatted milk and immunoblotted with pooled patients’ sera. Lane 1: 31 kDa protein (untreated), lane 2: 31 kDa protein after periodate treatment (B). Hemagglutination assay of 31 kDa protein, PHA (positive control) and PBS (negative control). Both 31 kDa and PHA formed uniform reddish color across the well with a minimum concentration of 15.62 µg/ml (C). Agglutination of human erythrocytes using purified protein and PHA (Sigma) (D).