<p>(A) Yeast cells (YS129 strain) transformed with pCM25 (<i>CUP1</i>-yEVenus-Cln2 -NLS) were cultured in the presence or absence of copper nitrate for 1 hr (middle panels) and 6 hrs (bottom panels). Cells with no copper nitrate are also shown in the upper panel. (B) Yeast cells (YS129) with pDHM57 (<i>ADH1</i>-yEVenus-Cln2 -NLS). (C) Yeast cells (YS129) with pRN1 (<i>ADH1</i>-yEVenus-NLS). Note that the fluorescence images in (B) and (C) were captured with the same exposure time. DIC: differential interference contrast; YFP: YFP filter channel for the detection of Venus fluorescence. .</p
<p>(<i>A</i>) Fluorescent and (<i>B</i>) Differential interference contrast (DIC) images of yeast ce...
<p>(A) Schematic illustration of the yeast strains expressing EYFP, ECFP, and mCherry. (B) Time-laps...
<p>Fluorescence output of chromosomal single-copy <i>yEGFP</i> under the control of <i>ADH1</i>p, <i...
<p>Yeast-based biosensors were either nontreated (NT, control) or exposed to increasing concentratio...
<p>Fluorescent microscope images of <sup>GFP</sup>S100A8 and <sup>GFP</sup>S100A9 (green) after (A) ...
<p>(A) Diagram of the reporter system. The <i>znf2</i>Δ mutant carries both the P<i><sub>GAL10</sub>...
<p>(A, C) Yeast that conditionally express each of the designated fusion proteins under the control ...
Part I. Yeast cells were exposed to 2μM α-factor and phenotypic changes were examined by time-lapse ...
Traditional use of the yeast two-hybrid (Y2H) system is primarily for identifying unknown protein in...
<p>(A) Schematic illustration of the yeast strains expressing EGFP and mCherry. Time-lapse images of...
(A) Yeast two-hybrid assay. DB:UCN was tested with all four versions of PDK1, the other UCN versions...
Bimodality in gene expression is thought to provide a high phenotypic heterogeneity that can be favo...
<p>(a) Bright field (BF) and fluorescence (FL) micrographs of yeast cells harboring empty control (p...
<p>(A) Diagrams of the <i>TXC</i> and the <i>TXC2</i> hybrid promoters driving <i>FD-GFP</i> express...
Expression of CTR genes based on qRT-PCR (A) or a CTR3 promoter fusion to gfp (B). Expression was ca...
<p>(<i>A</i>) Fluorescent and (<i>B</i>) Differential interference contrast (DIC) images of yeast ce...
<p>(A) Schematic illustration of the yeast strains expressing EYFP, ECFP, and mCherry. (B) Time-laps...
<p>Fluorescence output of chromosomal single-copy <i>yEGFP</i> under the control of <i>ADH1</i>p, <i...
<p>Yeast-based biosensors were either nontreated (NT, control) or exposed to increasing concentratio...
<p>Fluorescent microscope images of <sup>GFP</sup>S100A8 and <sup>GFP</sup>S100A9 (green) after (A) ...
<p>(A) Diagram of the reporter system. The <i>znf2</i>Δ mutant carries both the P<i><sub>GAL10</sub>...
<p>(A, C) Yeast that conditionally express each of the designated fusion proteins under the control ...
Part I. Yeast cells were exposed to 2μM α-factor and phenotypic changes were examined by time-lapse ...
Traditional use of the yeast two-hybrid (Y2H) system is primarily for identifying unknown protein in...
<p>(A) Schematic illustration of the yeast strains expressing EGFP and mCherry. Time-lapse images of...
(A) Yeast two-hybrid assay. DB:UCN was tested with all four versions of PDK1, the other UCN versions...
Bimodality in gene expression is thought to provide a high phenotypic heterogeneity that can be favo...
<p>(a) Bright field (BF) and fluorescence (FL) micrographs of yeast cells harboring empty control (p...
<p>(A) Diagrams of the <i>TXC</i> and the <i>TXC2</i> hybrid promoters driving <i>FD-GFP</i> express...
Expression of CTR genes based on qRT-PCR (A) or a CTR3 promoter fusion to gfp (B). Expression was ca...
<p>(<i>A</i>) Fluorescent and (<i>B</i>) Differential interference contrast (DIC) images of yeast ce...
<p>(A) Schematic illustration of the yeast strains expressing EYFP, ECFP, and mCherry. (B) Time-laps...
<p>Fluorescence output of chromosomal single-copy <i>yEGFP</i> under the control of <i>ADH1</i>p, <i...